In this era of personalized medicine, high-dimensional single-cell analysis is becoming critical to the study of human disease. Traditional approaches, like flow cytometry and RNAseq are typically performed in single-cell suspensions thus missing the spatial information and the tissue context. Tissue-imaging techniques like Immunofluorescence (IF) suffer from background fluorescence and spectral overlap, thus limiting the number of biomarkers that can be measured.
The advent of Imaging Mass Cytometry™ (IMC™) has enabled highly multiplexed immunohistochemistry of frozen and formalin-fixed, paraffin-embedded (FFPE) tissues using proven CyTOF technology. The technology helped uncover the central role of the immune system in a large variety of diseases including:
-Chronic inflammatory diseases
In this seminar, we will describe the principles of IMC™ and highlight groundbreaking research made possible with this technology.